Application of Bone Morphogenetic Protein 7 Enhanced the Osteogenic Differentiation and Mineralization of Bone Marrow-Derived Stem Cells Cultured on Deproteinized Bovine Bone

The growth of bone morphogenetic protein 7 (BMP-7) has been applied for tissue regeneration due to its osteoinductive properties. aim this research is analyze the enhancing effects BMP-7 on osteogenic differentiation and mineralization human marrow-derived stem cells cultured bovine particle. After were loaded onto graft material, their morphology was observed day 7. Viability assays based application fluorescent stains used qualitative analyses. Alkaline phosphatase activity Alizarin red staining assessment days 14. Next-generation mRNA sequencing evaluate global gene expression. Gene ontology pathway analysis propose underlying mechanism. Fibroblast-like attained with cells. shown be firmly attached Most produced an intense green fluorescence. relative cellular viability assay values groups at 0, 10, 100 ng/mL 0.295 ± 0.003, 0.250 0.002, 0.240 respectively (p < 0.05). significantly higher in concentration compared control 14 results showed when expression RUNX2 increased mitogen-activated kinase associated target genes. Overall, study shows that vitro increases alkaline phosphorylase culture deproteinized mineral. suggests combining factors scaffolds can have synergy differentiation.